Difference between revisions of "Part:BBa K1403009"
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Transform <i>E. coli</i> and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively. | Transform <i>E. coli</i> and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively. | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 15:18, 18 October 2014
Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette
- Constitutive promoter BBa_J23108
- Synthetic RBS
- CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
- Histidine Tag
Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively. Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 71
- 1000COMPATIBLE WITH RFC[1000]