Difference between revisions of "Part:BBa K1403006"
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<ul>
 
<ul>
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<li>Constitutive promoter BBa_J23108.
+
<li>Constitutive promoter BBa_J23108
 
<li>Synthetic RBS
 
<li>Synthetic RBS
 
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  
 
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  
−
<li>His Tag
+
<li>Histidine Tag
 
</ul>
 
</ul>
  
 
Transform <i>E. coli</i> for methyl jasmonate production.
 
Transform <i>E. coli</i> for methyl jasmonate production.
−
 
+
Requires jasmonic acid.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1403006 parameters</partinfo>
 
<partinfo>BBa_K1403006 parameters</partinfo>
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1 mM Jasmonic acid
+
 
 
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Revision as of 15:17, 18 October 2014

Jasmonic acid carboxyl methyltransferase (JMT) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: Jasmonic acid carboxyl methyltransferase (JMT)
  • Histidine Tag

Transform E. coli for methyl jasmonate production. Requires jasmonic acid. Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]