Difference between revisions of "Part:BBa K1403009"
Line 6: Line 6:
 
<li>Synthetic RBS
 
<li>Synthetic RBS
 
<li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
 
<li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
−
<li>His Tag
+
<li>Histidine Tag
 
</ul>
 
</ul>
  
−
Transform <i>E. coli</i> and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate, respectively.
+
Transform <i>E. coli</i> and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.
  
  

Revision as of 15:16, 18 October 2014

Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
  • Histidine Tag

Transform E. coli and grow in the presence of salicylic acid or benzoic acid for production of methyl salycilate or methyl benzoate respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]