Difference between revisions of "Part:BBa K1526007"

 
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<partinfo>BBa_K1526007 short</partinfo>
 
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===Usage and Biology===
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<p>Gene: mntH
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<p>Organism: Escherichia Coli
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<p>Protein: MntH transporter from NRAMP family
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<p>Function: NRAMP is a membrane divalent metal-ion transporter. In addition to iron and manganese, it also transports Cd ions into the cell.
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<p>Aim: We want to use the endogenous transporter NRAMP to take up the cadmium from the environment. The cadmium will then activate pYoda and set off the phytochelatin synthesis process.
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Expression: We can run assays measuring the concentration of cadmium in the environment. If NRAMP is active, the concentration of cadmium should decrease.
  
 
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Revision as of 15:07, 18 October 2014

LacRS + MntH

lacI + NRAMP

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1188
    Illegal BamHI site found at 2462
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 2358
  • 1000
    COMPATIBLE WITH RFC[1000]