Difference between revisions of "Part:BBa K1403012"
Line 5: Line 5:
 
<li>Constitutive promoter BBa_J23108
 
<li>Constitutive promoter BBa_J23108
 
<li>RBS BBa_B0030
 
<li>RBS BBa_B0030
−
<li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
+
<li>CDS: Alcohol acetyltransferase I (ATF1)
 
<li>Terminators BBa_B0010 & BBa_B0012
 
<li>Terminators BBa_B0010 & BBa_B0012
 
</ul>
 
</ul>

Revision as of 14:49, 18 October 2014

Alcohol acetyltransferase I (ATF1) expression cassette

  • Constitutive promoter BBa_J23108
  • RBS BBa_B0030
  • CDS: Alcohol acetyltransferase I (ATF1)
  • Terminators BBa_B0010 & BBa_B0012

Transform E. coli and grow in the presence of isoamyl alcohol to produce isoamyl acetate.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]