Difference between revisions of "Part:BBa K1403009"
Line 5: Line 5:
 
<li>Constitutive promoter BBa_J23108
 
<li>Constitutive promoter BBa_J23108
 
<li>Synthetic RBS
 
<li>Synthetic RBS
−
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  
+
<li>CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
 
<li>His Tag
 
<li>His Tag
 
</ul>
 
</ul>
  
−
Transform E. coli and grow in the prescence of jasmonic acid for methyl jasmonate biosynthesis.
+
Transform <i>E. coli</i> and grow in the presence of salicylic acid or benzoic acid for methyl salycilate or methyl benzoate production respectively.
  
  

Revision as of 14:14, 18 October 2014

Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1) expression cassette

  • Constitutive promoter BBa_J23108
  • Synthetic RBS
  • CDS: Benzoic acid/salicylic acid carboxyl methyltransferase 1 (BSMT1)
  • His Tag

Transform E. coli and grow in the presence of salicylic acid or benzoic acid for methyl salycilate or methyl benzoate production respectively.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]