Difference between revisions of "Part:BBa K1403006"

Line 3: Line 3:
  
 
<ul>
 
<ul>
<li>Constitutive promoter.
+
<li>Constitutive promoter BBa_J23108.
 
<li>Synthetic RBS
 
<li>Synthetic RBS
 
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  
 
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  

Revision as of 13:57, 18 October 2014

Jasmonic acid carboxyl methyltransferase (JMT) expression cassette

  • Constitutive promoter BBa_J23108.
  • Synthetic RBS
  • CDS: Jasmonic acid carboxyl methyltransferase (JMT)
  • His Tag

Transform E. coli for Methyl jasmonate biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]



1 mM Jasmonic acid