Difference between revisions of "Part:BBa K1403006"

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Line 5: Line 5:
 
<li>Constitutive promoter.
 
<li>Constitutive promoter.
 
<li>Synthetic RBS
 
<li>Synthetic RBS
−
<li>Jasmonic acid carboxyl methyltransferase (JMT)  
+
<li>CDS: Jasmonic acid carboxyl methyltransferase (JMT)  
 
<li>His Tag
 
<li>His Tag
 
</ul>
 
</ul>

Revision as of 12:48, 18 October 2014

Jasmonic acid carboxyl methyltransferase (JMT) expression cassette

  • Constitutive promoter.
  • Synthetic RBS
  • CDS: Jasmonic acid carboxyl methyltransferase (JMT)
  • His Tag

Transform E. coli for Methyl jasmonate biosynthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 222
    Illegal BamHI site found at 793
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 71
  • 1000
    COMPATIBLE WITH RFC[1000]



1 mM Jasmonic acid