Difference between revisions of "Part:BBa J01080:Experience"

Latest revision as of 09:29, 18 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_J01080

User Reviews

UNIQ3babec94a84af22a-partinfo-00000000-QINU

•••••

Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQ3babec94a84af22a-partinfo-00000002-QINU

•••

Hong_Kong_HKUST_2014

We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction to induce the Pbad promoter that regulates the key to the riboregulator system,, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.

For detailed information and figures, visit HKUST iGEM 2014 team wiki page http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization.

;

@@ Line 35: / Line 35: @@
 |width='60%' valign='top'|
-We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.
+We saw a strong repression after adding cis-repressing sequence 5’ of the RBS. After arabinose induction to induce the Pbad promoter that regulates the key to the riboregulator system,, we only saw 1.5 fold for 1% arabinose concentration (w/v) induction. For 2.5% we observed a 3 fold increase. The increase, however, only contributed to 0.3% of the fluorescence of the unrepressed controls.
-For more information, visit HKUST iGEM 2014 team wiki page [[http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization]].
+For detailed information and figures, visit HKUST iGEM 2014 team wiki page [[http://2014.igem.org/Team:Hong_Kong_HKUST/riboregulator/characterization]].
 |};