Difference between revisions of "Part:BBa K1431413"
| Line 1: | Line 1: | ||
| − | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1431413 short</partinfo> | <partinfo>BBa_K1431413 short</partinfo> | ||
| Line 5: | Line 4: | ||
we will complete this part later | we will complete this part later | ||
| − | + | This three plasmid(with BBa_K1431411 and BBa_K1431412) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. . | |
| + | |||
| + | However, although each one with different target-sequence, they still have plenty of components in common. You can see, all of them carry at least one section of target-sequences, and target-sequence for HBV1-HBV2 have two sections of target-sequence.This is because this part plays an important role in efficiency test, both for target-sequence binding gRNA and cut-off ability of CRISPR-Cas9. | ||
| + | |||
| + | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:54, 18 October 2014
target sequence2 for HBV
we will complete this part later
This three plasmid(with BBa_K1431411 and BBa_K1431412) carry the Target-sequence aim to check if the CRISPR-Cas9 system we create in human cell can work well, which might be the foundation for the further experiments. .
However, although each one with different target-sequence, they still have plenty of components in common. You can see, all of them carry at least one section of target-sequences, and target-sequence for HBV1-HBV2 have two sections of target-sequence.This is because this part plays an important role in efficiency test, both for target-sequence binding gRNA and cut-off ability of CRISPR-Cas9.
Usage and Biology
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]