Difference between revisions of "Part:BBa K1491016"
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The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm) | The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm) | ||
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Latest revision as of 03:52, 18 October 2014
Lac Inducible Codon Optimized KillerRed
Codon optimized KillerRed with WTLac promoter allows for induction of KillerRed expression with IPTG
It has been optimized for E. coli by eliminating the rare codons.
Characterization:
The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 583