Difference between revisions of "Part:BBa K1491018"
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<p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p> | <p>Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).</p> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 03:48, 18 October 2014
Lac Inducible Codon Optimized Supernova
Supernova is the monomeric version of KillerRed (BB_K1184000). Codon optimized Supernova with WTLac promoter allows for induction of Supernova expression with IPTG.
Of the three superoxide generators, Supernova had the biggest decrease in viability following photobleaching when compared to its control. It also had the largest decrease in fluorescence following photobleaching. Fluorescence was measured at (ex/em = 585nm/610nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 276
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]