Difference between revisions of "Part:BBa J36838:Design"
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There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains. | There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains. | ||
| − | + | Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts. | |
===Source=== | ===Source=== | ||
Latest revision as of 20:42, 29 October 2006
Lipoprotein signal peptide + Outer membrane protein A, aa 46-66
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
There are no spacer nucleotides between the part and the XbaI restriction site, or between the part and the SpeI restriction site, so that the mixed site would be 6bp long, and reading frame would be maintained between protein domains.
Also, the mixed site in between the parts is only six base pairs long, ACTAGA, without the spacer T or G in between parts.
Source
Assembly of BBa_J36835 and BBa_J36836