Difference between revisions of "Part:BBa K1418000"

Revision as of 03:35, 18 October 2014

Chlorophyllase (TaCHL from Triticum aestivum)

TaCHL is a chlorophyllase enzyme from Triticum aestivum, a common species of wheat. Primers were designed to use polymerase chain reaction (PCR) to amplify the TaCHL open reading frame from the pET28a vector that the gene was provided in. Since we were using the chlorophyllase gene in gene fusion applications, RFC 23 sequences were incorporated into the primers used for amplification. The amplified product was cloned into pSB1C3 and designated as BBa_K1418000. Primers were then designed and PCR was performed to remove the stop codon from TaCHL. This amplified product was cloned into pSB1C3 and is designated as BBa_K1418000.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 885
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 43
Illegal NgoMIV site found at 424
Illegal NgoMIV site found at 523
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 905

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1418000 short</partinfo>
-Chlorophyllase (TaCHL from <i>Triticum aestivum</i>)
+TaCHL is a chlorophyllase enzyme from <i>Triticum aestivum</i>, a common species of wheat. Primers were designed to use polymerase chain reaction (PCR) to amplify the TaCHL open reading frame from the pET28a vector that the gene was provided in.  Since we were using the chlorophyllase gene in gene fusion applications, RFC 23 sequences were incorporated into the primers used for amplification.  The amplified product was cloned into pSB1C3 and designated as BBa_K1418000.  Primers were then designed and PCR was performed to remove the stop codon from TaCHL. This amplified product was cloned into pSB1C3 and is designated as BBa_K1418000.
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