Difference between revisions of "Part:BBa K1444028"

 
 
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<partinfo>BBa_K1444028 short</partinfo>
 
<partinfo>BBa_K1444028 short</partinfo>
  
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Desc
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pET28b+ is a cloning vector commonly used in ''E. coli''. It is a high copy-number plasmid, containing a kanamycin resistance cassette. This backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard <partinfo>pSB1C3</partinfo> cloning vector.
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This plasmid came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 03:00, 18 October 2014

pET28b+ cloning vector from BioBasic

pET28b+ is a cloning vector commonly used in E. coli. It is a high copy-number plasmid, containing a kanamycin resistance cassette. This backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard pSB1C3 cloning vector.

This plasmid came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal NheI site found at 230
    Illegal NotI site found at 165
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal BglII site found at 400
    Illegal BamHI site found at 198
    Illegal XhoI site found at 158
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal EcoRI site found at 192
    Illegal XbaI site found at 334
    Illegal NgoMIV site found at 432
    Illegal NgoMIV site found at 2020
    Illegal NgoMIV site found at 2180
    Illegal NgoMIV site found at 5227
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal SapI site found at 3100