Difference between revisions of "Part:BBa K1366106"

Revision as of 02:59, 18 October 2014

Production of survivin siRNA under a hTERT promoter (C7)

This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.

In the next image it is presented the restriction digestion analysis of some biobricks:

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 897

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1366106 short</partinfo>
 This construct will be used to characterize the transfection of an effector of death (Survivin siRNA) in mammalian cells mediated by bacteriophages. It contains the hTERT promoter, specific for cancerous cells lines. The construct will be contained in a phagemid with de f1 origin of replication (for single stranded DNA), and it is intended to replace the M13 genome.
+In the next image it is presented the restriction digestion analysis of some biobricks:
+https://static.igem.org/mediawiki/2014/2/2b/Gel_psb1c3.png
 <!-- Add more about the biology of this part here