Difference between revisions of "Part:BBa K1444027"
 
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pUC57 is a derivative of pUC19, and a commonly used cloning vector in ''E. coli''. It has a high copy-number origin, contains an ampicillin resistance cassette, and blue/white colony selection around the multiple cloning site. This plasmid backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard <partinfo>pSB1C3</partinfo> cloning vector.
 
pUC57 is a derivative of pUC19, and a commonly used cloning vector in ''E. coli''. It has a high copy-number origin, contains an ampicillin resistance cassette, and blue/white colony selection around the multiple cloning site. This plasmid backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard <partinfo>pSB1C3</partinfo> cloning vector.
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This plasmid came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:54, 18 October 2014

pUC57 cloning vector from BioBasic

pUC57 is a derivative of pUC19, and a commonly used cloning vector in E. coli. It has a high copy-number origin, contains an ampicillin resistance cassette, and blue/white colony selection around the multiple cloning site. This plasmid backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard pSB1C3 cloning vector.

This plasmid came from the gene synthesis services offered by [http://store.biobasic.com/gene-synthesis/ BioBasic].


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1727
    Illegal SapI site found at 644