Difference between revisions of "Part:BBa J31006:Design"
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===Design Notes=== | ===Design Notes=== | ||
− | + | BBa J31006 was cloned into vector pSB1A2. The Biobricks on this part are not wild type, but the cut sites are still viable. | |
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− | The Biobricks on this part are not wild type, but the cut sites are still viable. | + | |
{| width="800px" cellspacing="5" | {| width="800px" cellspacing="5" | ||
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| style="width:180px" | '''BBa_J31001 Cloning Sites''' | | style="width:180px" | '''BBa_J31001 Cloning Sites''' | ||
− | | style="background:lightgrey" |<font face="courier">5'--GAATTC GCGGCCGC T TCTAGA <font color='blue'>*</font> -- | + | | style="background:lightgrey" |<font face="courier">5'--GAATTC GCGGCCGC T TCTAGA <font color='blue'>*</font> --Tet coding-- <font color='purple'>*</font> ACTAGT A GCGGCCG CTGCAG--<br>3'--CTTAAG CGCCGGCG A AGATCT <font color='blue'>*</font> -------------- <font color='purple'>*</font> TGATCA T CGCCGGC GACGTC--</font><br><br> |
'''Prefix'''<br>There is <font color='blue'>no G spacer (*)</font> between the XbaI and the Hin coding region.<br> | '''Prefix'''<br>There is <font color='blue'>no G spacer (*)</font> between the XbaI and the Hin coding region.<br> | ||
− | '''Suffix'''<br>There is <font color='purple'>no T spacer (*)</font> between the Hin coding region and the SpeI site. | + | '''Suffix'''<br>There is <font color='purple'>no T spacer (*)</font> between the Hin coding region and the SpeI site. |
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===Source=== | ===Source=== | ||
− | + | The tetracycline resistance coding region was PCR amplified from <partinfo>pSB1AT3</partinfo>. | |
===References=== | ===References=== | ||
− | [https://dspace.mit.edu/handle/1721.1/21168| Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks] | + | * [https://dspace.mit.edu/handle/1721.1/21168| Knight, Tom. Idempotent Vector Design for Standard Assembly of Biobricks] |
Revision as of 17:14, 29 October 2006
tetracycline resistance protein TetA(C) (backwards) [cf. BBa_J31007]
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1043
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 897
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 343
Illegal NgoMIV site found at 503
Illegal NgoMIV site found at 871 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
BBa J31006 was cloned into vector pSB1A2. The Biobricks on this part are not wild type, but the cut sites are still viable.
Standard BioBrick Cloning Sites (Knight) | 5'--GAATTC GCGGCCGC T TCTAGA G ----insert---- T ACTAGT A GCGGCCG CTGCAG-- 3'--CTTAAG CGCCGGCG A AGATCT C -------------- A TGATCA T CGCCGGC GACGTC-- |
BBa_J31001 Cloning Sites | 5'--GAATTC GCGGCCGC T TCTAGA * --Tet coding-- * ACTAGT A GCGGCCG CTGCAG-- 3'--CTTAAG CGCCGGCG A AGATCT * -------------- * TGATCA T CGCCGGC GACGTC-- Prefix |
Source
The tetracycline resistance coding region was PCR amplified from pSB1AT3.