Difference between revisions of "Part:BBa K1444027"

 
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<partinfo>BBa_K1444027 short</partinfo>
 
<partinfo>BBa_K1444027 short</partinfo>
  
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Descr
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pUC57 is a derivative of pUC19, and a commonly used cloning vector in ''E. coli''. It has a high copy-number origin, contains an ampicillin resistance cassette, and blue/white colony selection around the multiple cloning site. This plasmid backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard <partinfo>pSB1C3</partinfo> cloning vector.
  
 
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Revision as of 02:49, 18 October 2014

pUC57 cloning vector from BioBasic

pUC57 is a derivative of pUC19, and a commonly used cloning vector in E. coli. It has a high copy-number origin, contains an ampicillin resistance cassette, and blue/white colony selection around the multiple cloning site. This plasmid backbone was used by the 2014 University of Calgary team for a series of repressible promoters, as our experience with these parts suggested that they were unstable in the standard pSB1C3 cloning vector.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BsaI.rc site found at 1727
    Illegal SapI site found at 644