Difference between revisions of "Part:BBa K1429002:Design"

 
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We had to make sure that we could do the transformation properly.
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We started with the FPB-20-444 plasmid from DNA 2.0 which contains an "IP-Free" synthetic non-Aequorea CFP coding region downstream from a ribosome binding site (AGGAGG ). PCR primers were designed complementary to sequences flanking the RBS-CFP region, with a "tail" that added the biobrick prefix/suffix. The PCR product was digested with EcoR1 and Pst1 and ligated into the pSB1C3 plasmid backbone.
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===Source===
 
===Source===

Revision as of 02:36, 18 October 2014


Cyan Fluorescent Protein (CFP) "Cindy Lou" coding region, intellectual property-free


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 74
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2


Design Notes

We started with the FPB-20-444 plasmid from DNA 2.0 which contains an "IP-Free" synthetic non-Aequorea CFP coding region downstream from a ribosome binding site (AGGAGG ). PCR primers were designed complementary to sequences flanking the RBS-CFP region, with a "tail" that added the biobrick prefix/suffix. The PCR product was digested with EcoR1 and Pst1 and ligated into the pSB1C3 plasmid backbone.

Source

I think that CFP is a variant of GFP.

References