Difference between revisions of "Part:BBa K1462520"

 
 
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<partinfo>BBa_K1462520 short</partinfo>
 
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This part is the final device including the BGY fluorescent protein with the ligand behind.  We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein. And we used the confocal to make sure whether the scaffold protein work and the enzyme can be bind on the site of it.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:35, 18 October 2014

GAL1+YFP+SH3-lig+ADH1+GAL1+GFP+PDZ-lig+ADH1+GAL1+BFP+GBD-lig+ADH1

This part is the final device including the BGY fluorescent protein with the ligand behind. We planned to transmit this device to yeast with the different scale group of GBD,PDZ and SH3 scaffold protein. And we used the confocal to make sure whether the scaffold protein work and the enzyme can be bind on the site of it.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
    Illegal AgeI site found at 1755
    Illegal AgeI site found at 3300
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2804