Difference between revisions of "Part:BBa K1365006"

 
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<partinfo>BBa_K1365006 short</partinfo>
 
<partinfo>BBa_K1365006 short</partinfo>
  
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Coding part for the genes NisR and NisK.
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===Usage and Biology===
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This BioBrick contains the coding parts for the genes NisR and NisK. NisK is a membrane-associated protein kinase that phosphorylates NisR in response to nisin in the environment. NisR is a regulatory protein, regulating promoters like PNisA and PNisI.
  
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NisK is a membrane-associated protein kinase that phosphorylates NisR in response to enviromental signals. NisR is a regulatory protein, regulating PNisI and other promoters.
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Together with the genes NisA, NisB, NisC, NisP they are responsible for producing the lantibiotic nisin in ''Lactococcus lactis'', see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.<sup>1</sup>
  
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Together with the genes NisA, NisB, NisC, NisP they are responsible for producing the lantibiotic nisin in Lactococcus lactis.  
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[[File:Nisin_casette.art.png]]
  
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<!-- Add more about the biology of this part here
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Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.<sup>2</sup>
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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===Sequence and features===
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<span class='h3bb'></span>
 
<partinfo>BBa_K1365006 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1365006 SequenceAndFeatures</partinfo>
  
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===References===
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1. Cheigh, C. I. and Pyun, Y.R. (2005) Nisin biosynthesis and its properties. ''Biotechnol. Lett.'' 27: 1641-1648<br>
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2. Zhou, H. et al. (2014) Mechanisms of nisin resistance in Gram-positive bacteria. ''Ann. Microbiol.'' 64: 413-420 
  
 
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Revision as of 02:34, 18 October 2014

NisR and NisK

Usage and Biology

This BioBrick contains the coding parts for the genes NisR and NisK. NisK is a membrane-associated protein kinase that phosphorylates NisR in response to nisin in the environment. NisR is a regulatory protein, regulating promoters like PNisA and PNisI.

Together with the genes NisA, NisB, NisC, NisP they are responsible for producing the lantibiotic nisin in Lactococcus lactis, see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.1

Nisin casette.art.png

Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.2


Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Cheigh, C. I. and Pyun, Y.R. (2005) Nisin biosynthesis and its properties. Biotechnol. Lett. 27: 1641-1648
2. Zhou, H. et al. (2014) Mechanisms of nisin resistance in Gram-positive bacteria. Ann. Microbiol. 64: 413-420