Difference between revisions of "Part:BBa K1462500"

 
 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1462500 short</partinfo>
 
<partinfo>BBa_K1462500 short</partinfo>
  
−
ddd
+
This is the composite part, consist of the GAL1 promoter, GFP fusing with the PDZ ligand and the ADH1 terminator. Now we made this<br/> device to check whether the ligand owns the binding funtion or not. It helped us to affirm the scaffold protein and it's ligands can work.<br/>Besides, it's induced by the existence of galactose.<br/>
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 02:31, 18 October 2014

GAL1+GFP+PDZ-lig+ADH1

This is the composite part, consist of the GAL1 promoter, GFP fusing with the PDZ ligand and the ADH1 terminator. Now we made this
device to check whether the ligand owns the binding funtion or not. It helped us to affirm the scaffold protein and it's ligands can work.
Besides, it's induced by the existence of galactose.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1199