Difference between revisions of "Part:BBa K1429001:Experience"

Latest revision as of 02:26, 18 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1429001

We have put this biobrick under the control of the T7 promoter by cloning the biobrick part into pUC19. The expression of the RFPRudolph can be seen in the photograph. It is clone RFP2:

The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 553 and 570 respectively. They did, confirming the protein was the correct one.

User Reviews

UNIQ6282e0d1db79fc46-partinfo-00000000-QINU UNIQ6282e0d1db79fc46-partinfo-00000001-QINU

@@ Line 11: / Line 11: @@
 https://static.igem.org/mediawiki/2014/d/d7/20141015_174513.jpg
-<p>The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 550 and 563 respectively. They did, confirming the protein was the correct one. </p>
+<p>The lysis buffer from the Carolina Biological kit "Purification of Green Fluorescent Protein" was used to lyse cells from an overnight 2mL culture of E. coli K-12 Top10 cells containing pUC19 with this biobrick. The lysate was analyzed by fluorimetry to determine if the excitation and emission wavelengths matched the reported ones of 553 and 570 respectively. They did, confirming the protein was the correct one. </p>
 https://static.igem.org/mediawiki/2014/6/68/RFP.jpg