Difference between revisions of "Part:BBa K1498000:Experience"
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===Applications of BBa_K1498000===
 
===Applications of BBa_K1498000===
  
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Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 39.4 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity.
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Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.
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In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).
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As a protease gelatin degredation should also be observed.
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===User Reviews===
 
===User Reviews===

Revision as of 02:18, 18 October 2014

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Applications of BBa_K1498000

Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of around 36 kDa was visibly overexpressed -this size is smaller than the unprocessed keratinase, but larger than the fully processed protein suggesting that a pre-processed version of the protein was present. However only the processed form of the protein will exhibit activity.

User Reviews

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