Difference between revisions of "Part:BBa K1365003:Design"

 
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===References===
 
===References===
 +
Trmčić, A. et al. (2011) Complete nisin A gene cluster from ''Lactococcus lactis'' M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. ''Genes Genom.'' 33: 217-221

Revision as of 02:18, 18 October 2014


NisC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used primers:

forward - 5'-GTTTCTTCGAATTCGCGGCCGCTTCTAGATGAATAAAAAAAATATAAA-3'

reverse - 5'-GTTTCTTCCTGCAGCGGCCGCTACTAGTATTATCATTTCCTCTTCCCTC-3'

to PCR NisC from the genome and add the BioBrick prefix and suffix.


Source

Obtained by PCR on the genome of Lactococcus lactis NZ9700, a nisin producing strain.

References

Trmčić, A. et al. (2011) Complete nisin A gene cluster from Lactococcus lactis M78 (HM219853) – obtaining the nucleic acid sequence and comparing it to other published nisin sequences. Genes Genom. 33: 217-221