Difference between revisions of "Part:BBa K1491020"

Revision as of 02:17, 18 October 2014

Constitutive Codon Optimized Killer Red

Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).

It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 418

@@ Line 4: / Line 4: @@
 <p>Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).</p>
-<p>This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.</p>
+<p>It has been optimized for E. coli by eliminating the rare codons.</p>
 <p><b>Characterization:</b></p>
 The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)