Difference between revisions of "Part:BBa K1491020"

 
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<partinfo>BBa_K1491020 short</partinfo>
 
<partinfo>BBa_K1491020 short</partinfo>
  
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Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).
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<p>Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).</p>
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<p>This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.</p>
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<p><b>Characterization:</b></p>
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The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)
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https://static.igem.org/mediawiki/2014/thumb/b/b0/Colonies.jpg/800px-Colonies.jpg
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<hr>
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https://static.igem.org/mediawiki/2014/thumb/e/e0/RFU.jpg/736px-RFU.jpg
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 02:17, 18 October 2014

Constitutive Codon Optimized Killer Red

Codon optimized version of superoxide generator Killer Red (BBa_K1491015) with a constitutive promoter (BBa_J23100) and a ribosome binding site (BBa_B0034).

This part is the codon optimized version of KillerRed (BBa_K1184000). It has been optimized for E. coli by eliminating the rare codons.

Characterization:

The codon optimized KillerRed and codon optimized monomeric form (SuperNova) were characterized and compared to the original KillerRed using photobleaching and viability assays. It was observed to have a significant decrease in RFU in response to photobleaching, in comparison to the original KillerRed. Fluorescence was measured at (ex/em = 585nm/610nm)

800px-Colonies.jpg

736px-RFU.jpg

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 418