Difference between revisions of "Part:BBa K1365003"

 
 
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<partinfo>BBa_K1365003 short</partinfo>
 
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NisC catalyzes the ring formation in the nisin precursor protein. Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis.
 
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===Usage and Biology===
 
===Usage and Biology===
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NisC catalyzes the ring formation in the nisin precursor protein NisA (BBa_K1365000). Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in ''Lactococcus lactis''. see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.<sup>1</sup>
  
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[[File:Nisin_casette.art.png]]
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<span class='h3bb'>Sequence and Features</span>
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Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.<sup>2</sup>
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===Sequence and features===
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<span class='h3bb'></span>
 
<partinfo>BBa_K1365003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1365003 SequenceAndFeatures</partinfo>
  
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===References===
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1. Cheigh, C. I. and Pyun, Y.R. (2005) Nisin biosynthesis and its properties. ''Biotechnol. Lett.'' 27: 1641-1648<br>
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2. Zhou, H. et al. (2014) Mechanisms of nisin resistance in Gram-positive bacteria. ''Ann. Microbiol.'' 64: 413-420 
  
 
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Latest revision as of 02:16, 18 October 2014

NisC

Usage and Biology

NisC catalyzes the ring formation in the nisin precursor protein NisA (BBa_K1365000). Together with the genes NisA, NisB, NisT, NisP, NisR and NisK it is responsible for producing the lantibiotic nisin in Lactococcus lactis. see the figure below. The NisA protein is first modified and then transported out of the cell. The serines and threonines of NisA are dehydrated by NisB and then the precursor is cyclized by NisC. After this process, the precursor is transported out of the cell (2). Here, the lead peptide is cut off by NisP (3) and the mature nisin is formed.1

Nisin casette.art.png

Nisin is a lantibiotic, an bacteriocidal peptide. Nisin inhibits the growth of a broad range of Gram positive bacteria, of which many are spoilage bacteria or pathogens. Nisin is therefore extensively used in the food industry as a preservative. Nisin forms pores in the membrane of the bacteria it kills and inhibits the peptidoglycan synthesis.2


Sequence and features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Cheigh, C. I. and Pyun, Y.R. (2005) Nisin biosynthesis and its properties. Biotechnol. Lett. 27: 1641-1648
2. Zhou, H. et al. (2014) Mechanisms of nisin resistance in Gram-positive bacteria. Ann. Microbiol. 64: 413-420