Difference between revisions of "Part:BBa K1431834:Experience"

 
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
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how you used this part and how it worked out.
 
  
 
===Applications of BBa_K1431834===
 
===Applications of BBa_K1431834===
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After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431834 and BBa_K1431824 whose RBS is BBa_B0031, a relatively weak RBS.
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==LB agar plate growth comparison==
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We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.
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JA is J23100,JB is J23106.
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At 00:30 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/58/SUSTC_31_916-1.jpg</center>
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At 1:50  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/94/SUSTC_31_916-2.jpg</center>
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At 3:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f6/SUSTC_31_916-3.jpg</center>
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At 6:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/56/SUSTC_31_916-4.jpg</center>
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At 7:40  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/c3/SUSTC_31_916-5.jpg</center>
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At 8:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/94/SUSTC_31_916-6.jpg</center>
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At 10:00 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/0/0b/SUSTC_31_916-7.jpg</center>
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At 11:20 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f3/SUSTC_31_916-8.jpg</center>
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==LB broth growth comparison==
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We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol.
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Then we isolated single colonies from the plates.
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At 00:50 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/fb/SUSTC_31_916_L-1.jpg</center>
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At 02:45 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/33/SUSTC_31_916_L-2.jpg</center>
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At 04:55 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/0/05/SUSTC_31_916_L-3.jpg </center>
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At 22:40 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/95/SUSTC_31_916_L-4.jpg </center>
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==Conclusion==
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'''With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.
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===User Reviews===
 
===User Reviews===

Revision as of 02:16, 18 October 2014

Applications of BBa_K1431834

After we constructed this part, we tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431834 and BBa_K1431824 whose RBS is BBa_B0031, a relatively weak RBS.

LB agar plate growth comparison

We spread the plate for BL21 at 10:00am on 15th, Oct and DH5α at 11:00am on 15th, Oct. The plate contains 34ug/ml chloramphenicol. Following pictures were taken every several hours after placing the plates into a 37℃ incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

SUSTC_31_916-1.jpg


At 1:50 (16th,Oct.)

SUSTC_31_916-2.jpg


At 3:00 (16th,Oct.)

SUSTC_31_916-3.jpg


At 6:00 (16th,Oct.)

SUSTC_31_916-4.jpg


At 7:40 (16th,Oct.)

SUSTC_31_916-5.jpg


At 8:00 (16th,Oct.)

SUSTC_31_916-6.jpg


At 10:00 (16th,Oct.)

SUSTC_31_916-7.jpg


At 11:20 (16th,Oct.)

SUSTC_31_916-8.jpg

LB broth growth comparison

We isolated single colonies from the plates and incubate in 2ml LB broth with 34ug/ml chloramphenicol. Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC_31_916_L-1.jpg


At 02:45 (17th,Oct.)

SUSTC_31_916_L-2.jpg


At 04:55 (17th,Oct.)

SUSTC_31_916_L-3.jpg


At 22:40 (17th,Oct.)

SUSTC_31_916_L-4.jpg

Conclusion

With observation, expression of BBa_K1431834 is stronger than BBa_K1431824.


User Reviews

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