Difference between revisions of "Part:BBa K1378022"
Line 6: Line 6:
 
</html>
 
</html>
 
MlrA coding sequence fused with PelB secretion signal peptide.
 
MlrA coding sequence fused with PelB secretion signal peptide.
 +
 +
MlrA is a 28kDa protease found in ''Sphingomonas'' sp which can cleavage microcystins(MCs).
 +
 +
MlrA is one part of the gene cluster responsible for the ability of MC degradation. The cluster includes four ORFs, ''mlrA, mlrB, mlrC and mlrD'', which can hydrolyze MCs and facilitate absorption of the products as carbon source.
 +
MlrA is sometimes referred as a metalprotease by inhibitor studies.
 +
<html>
 +
<p>MlrA can cleavage the Adda-Arg bond and causes ring opening.(Fig. 1) The first-step linearized product shows much weaker hepatoxin compared with MCs. In the experiment of mouse bioassay, up to 250 mg/kg of linearized MC-LR shows no toxicity to mouse, much higher than 50% lethal dose 50mg/kg of cyclic MC-LR. Furthermore, the linearization also raise the median inhibition concentration to 95nM, around 160 times higher than original 0.6nM. <sup>[1]</sup> </p>
 +
 +
<figure><img src="https://static.igem.org/mediawiki/2014/0/05/Peking2014jyj_2.png"/><figcaption>Fig. 1 First step of biodegradation of MC-LR. MlrA mediates breaking peptide bond between Adda and Arg, which leads to significant decrease of toxicity.<sup>[1]</sup></figcaption></figure>
 +
</html>
 +
 +
  
  

Revision as of 02:11, 18 October 2014

PelB-MlrA

MlrA coding sequence fused with PelB secretion signal peptide.

MlrA is a 28kDa protease found in Sphingomonas sp which can cleavage microcystins(MCs).

MlrA is one part of the gene cluster responsible for the ability of MC degradation. The cluster includes four ORFs, mlrA, mlrB, mlrC and mlrD, which can hydrolyze MCs and facilitate absorption of the products as carbon source. MlrA is sometimes referred as a metalprotease by inhibitor studies.

MlrA can cleavage the Adda-Arg bond and causes ring opening.(Fig. 1) The first-step linearized product shows much weaker hepatoxin compared with MCs. In the experiment of mouse bioassay, up to 250 mg/kg of linearized MC-LR shows no toxicity to mouse, much higher than 50% lethal dose 50mg/kg of cyclic MC-LR. Furthermore, the linearization also raise the median inhibition concentration to 95nM, around 160 times higher than original 0.6nM. [1]

Fig. 1 First step of biodegradation of MC-LR. MlrA mediates breaking peptide bond between Adda and Arg, which leads to significant decrease of toxicity.[1]



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 54
    Illegal AgeI site found at 310
    Illegal AgeI site found at 439
  • 1000
    COMPATIBLE WITH RFC[1000]