Difference between revisions of "Part:BBa K1372000"
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<partinfo>BBa_K1372000 short</partinfo> | <partinfo>BBa_K1372000 short</partinfo> | ||
− | This | + | This Biobrick is the result of the assembly of two Biobrick: |
- [https://parts.igem.org/Part:BBa_J61051 BBa_J61051] for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). | - [https://parts.igem.org/Part:BBa_J61051 BBa_J61051] for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). |
Latest revision as of 02:10, 18 October 2014
salicylate inducible suppressing system
This Biobrick is the result of the assembly of two Biobrick:
- BBa_J61051 for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
- BBa_K228001: for the transcription of SupD-tRNA.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1312
Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]