Difference between revisions of "Part:BBa K1520520"

Revision as of 02:06, 18 October 2014

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter

PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter It is part of our test of suicide circuit by gold ions, BBa_K1520509 [1].

this is part of our part BBa_K1520509 [2] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.

The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 548
1000
COMPATIBLE WITH RFC[1000]

@@ Line 5: / Line 5: @@
 It is part of our test of suicide circuit by gold ions, BBa_K1520509 [https://parts.igem.org/Part:BBa_K1520509].
+[[File:suicidewithoutgold.jpg]]
+this is part of our part BBa_K1520509 [https://parts.igem.org/Part:BBa_K1520509] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.
+[[File:k1520509.jpg]]
+From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.
+The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
 since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.