Difference between revisions of "Part:BBa K1520519"
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this is part of our part BBa_K1520509 [https://parts.igem.org/Part:BBa_K1520509] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work. | this is part of our part BBa_K1520509 [https://parts.igem.org/Part:BBa_K1520509] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work. | ||
| − | [[File: | + | [[File:k1520509.jpg]] |
| − | From left to right, Marker( | + | From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter. |
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers. | ||
Latest revision as of 01:53, 18 October 2014
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter
PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter. If gold ions exist, rfp will not be produced.
this is part of our part BBa_K1520509 [1] and the picture is also part of BBa_K1520509, since BBa_K1520509 is based on this one and our result shows it works, this one would definitely work.
From left to right, Marker(1Kb), PgolTS-golB-PgolB-tetR-Ter, PgolTS-golB-PgolB-tetR-Ter-Ptet-rbs-rfp-Ter.
The substances of electrophoresis are made from PCR of plasmid with VF2 and VR as their primers.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 548
- 1000COMPATIBLE WITH RFC[1000]