Difference between revisions of "Part:BBa J58103"
 
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<partinfo>BBa_J58103 short</partinfo>
 
<partinfo>BBa_J58103 short</partinfo>
  
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Many signalling cellular processes occur using a phosphorylation mechanism. In order to assemble a transmembranal pathway, that frees a phosphore, with a genetic network within the cell, we can use this promoter (R0083). That promoter is regulated positively (activated) by the transcription factor OmpR-P, and it contains only one operator in its sequence, whereas in the natural promoter (R0082) there are three. We put a well known protein downstream: GFP (E0040) with a RBS (B0034) and a Terminator (B0015). This composite part will be very useful to characterize many cellular biosensors, jointly with the part J58102.
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We have assembled promoter <partinfo>R0083</partinfo> to GFP for characterization purposes. That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated), and it contains only one operator in its sequence, whereas in the natural promoter <partinfo>R0082</partinfo> there are three.  
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The deep components are as follows:<partinfo>J58103 DeepComponents</partinfo> This composite part will be very useful to characterize many cellular biosensors, jointly with the part <partinfo>J58102</partinfo>.
  
 
'''By Valencia iGEM 2006'''
 
'''By Valencia iGEM 2006'''
  
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A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).
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''A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).''
  
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1 Universidad Politecnica de Valencia, Spain
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''1 Universidad Politecnica de Valencia, Spain''
  
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2 Universitat de Valencia, Spain
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''2 Universitat de Valencia, Spain''
  
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3 Ecole Polytechnique, France
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''3 Ecole Polytechnique, France''
  
  

Latest revision as of 08:31, 29 October 2006

Mutated promoter activated by OmpR-P with the reporter GFP

We have assembled promoter BBa_R0083 to GFP for characterization purposes. That promoter is regulated positively (activated) by the transcription factor OmpR-P (phosphorylated), and it contains only one operator in its sequence, whereas in the natural promoter BBa_R0082 there are three.

The deep components are as follows:
OmpR
R0083
B0034		GFP
E0040
B0010
B0012
This composite part will be very useful to characterize many cellular biosensors, jointly with the part BBa_J58102.

By Valencia iGEM 2006

A. Aparici (2), MC. Aroca (2), J. Carrera (1), C. Edo (1,2), G. Fuertes (2), D. Jimenez (2), C. Mata (2), JV. Medrano (2), A. Montagud (2), C. Navarrete (2), E. Navarro (1), G. Rodrigo (1), M. Baguena (1), P. Tortosa (3), P. Fernandez-de-Cordoba (1), A. Ferrando (2), J. Salgado (2), J. Urchueguia (1), A. Jaramillo (3).

1 Universidad Politecnica de Valencia, Spain

2 Universitat de Valencia, Spain

3 Ecole Polytechnique, France


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 748