Difference between revisions of "Part:BBa K1491036"
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<p>https://static.igem.org/mediawiki/2014/9/9c/Screen_Shot_2014-10-17_at_8.47.08_PM.png</p> | <p>https://static.igem.org/mediawiki/2014/9/9c/Screen_Shot_2014-10-17_at_8.47.08_PM.png</p> | ||
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| + | <b>TECAN Settings to Detect RFP Signal from the Sensor:</b> | ||
| + | <p>emission: 585 nm</p> | ||
| + | <p>excitation: 607</p> | ||
| + | <p>bandwidth: 10nm</p> | ||
| + | <p>gain: 175</p> | ||
| + | |||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Revision as of 01:49, 18 October 2014
Constitutive Dead ER Intein T7 RNAP with YFPH
T7 RNA polymerase that contains the dead version estrogen receptor intein with a constitutive promoter. YFP is added to the end of the plasmid so that the amount of T7 RNA polymerase that is produced can be quantified through fluorescence analysis of YFP. Used as a control.
Characterization
The fluorescence of the sensor and its controls were measure in the red and yellow channels with and without the presence of beta-estradiol over several time points.
*The magnitude of fluorescence of T7 RNA polymerase between the red and yellow channels was so large that they are presented on two different graphs.
TECAN Settings to Detect RFP Signal from the Sensor:
emission: 585 nm
excitation: 607
bandwidth: 10nm
gain: 175
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2525
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 2950
Illegal AgeI site found at 2914 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2425