Difference between revisions of "Part:BBa K1491012"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1491012 short</partinfo> | <partinfo>BBa_K1491012 short</partinfo> | ||
His tagged SuperYFP2 with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | His tagged SuperYFP2 with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | ||
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| + | <hr> | ||
| + | <p> | ||
| + | We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). YFP had a very high signal-to-noise ratio in MACH cells and a low signal-to-noise ratio in Top10 cells relative to other fluorescent proteins. YFP was measured at (ex/em = 514nm/527nm). | ||
| + | </p> | ||
| + | https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png | ||
| + | https://static.igem.org/mediawiki/2014/thumb/9/9f/Top10_Best.jpg/591px-Top10_Best.jpg | ||
| + | <hr> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 01:41, 18 October 2014
WTLac-RBS-YFP-His
His tagged SuperYFP2 with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence.
We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). YFP had a very high signal-to-noise ratio in MACH cells and a low signal-to-noise ratio in Top10 cells relative to other fluorescent proteins. YFP was measured at (ex/em = 514nm/527nm).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]