Difference between revisions of "Part:BBa K1491011"
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His tagged eGFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | His tagged eGFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence. | ||
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https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png | https://static.igem.org/mediawiki/2014/thumb/c/c1/FP_MACH.png/591px-FP_MACH.png | ||
https://static.igem.org/mediawiki/2014/thumb/9/9f/Top10_Best.jpg/591px-Top10_Best.jpg | https://static.igem.org/mediawiki/2014/thumb/9/9f/Top10_Best.jpg/591px-Top10_Best.jpg | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 01:40, 18 October 2014
WTLac-RBS-GFP-His
His tagged eGFP with a strong RBS. WT Lac promoter allows for Lac induction of fluorescence.
We characterized a set of fluorescent proteins consisting of BFP. GFP, YFP, OFP, and RFP. We calculated the signal-to-noise ratio of all the proteins in two different cell lines (MACH and Top10). GFP had one the lowest signal-to-noise ratios in both cell lines. GFP was measured at (ex/em = 488nm/509nm).
</p>
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]