Difference between revisions of "Part:BBa J23006"

Revision as of 04:50, 29 October 2006

Part-making plasmid with Ptet-XbaI-[biobrick]-SpeI-PstI

pSB1A2-derived plasmid for constructing basic biobrick parts under the control of an external promoter. Insertion of parts between the XbaI and SpeI (or) PstI sites places the part under the control of the part r0040 (Tet) promoter. The resulting plasmid retains the normal sequence context of a r0040.newpart composite with one point mutation changing the ACTAGA linker to TCTAGA.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Plasmid lacks a prefix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350
12
INCOMPATIBLE WITH RFC[12]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal SpeI site found at 2
Illegal PstI site found at 16
Illegal NotI site found at 9
Illegal NotI site found at 2142
21
INCOMPATIBLE WITH RFC[21]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
23
INCOMPATIBLE WITH RFC[23]
Plasmid lacks a prefix.
Illegal suffix found at 2
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350
25
INCOMPATIBLE WITH RFC[25]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal EcoRI site found at 2136
Illegal XbaI site found at 2350
Illegal SpeI site found at 2
Illegal PstI site found at 16
1000
INCOMPATIBLE WITH RFC[1000]
Plasmid lacks a prefix.
Plasmid lacks a suffix.
Illegal BsaI.rc site found at 1175

@@ Line 4: / Line 4: @@
 [[Image:Berkley2006-pJ23006Map.GIF |center|400px]]
-pSB1A3-derived plasmid for constructing basic biobrick parts under the control of an external promoter.  Insertion of parts between the XbaI and SpeI (or) PstI sites places the part under the control of the part r0040 (Tet) promoter.  The resulting plasmid retains the normal sequence context of a r0040.newpart composite with one point mutation changing the ACTAGA linker to  TCTAGA.
+pSB1A2-derived plasmid for constructing basic biobrick parts under the control of an external promoter.  Insertion of parts between the XbaI and SpeI (or) PstI sites places the part under the control of the part r0040 (Tet) promoter.  The resulting plasmid retains the normal sequence context of a r0040.newpart composite with one point mutation changing the ACTAGA linker to  TCTAGA.
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