Difference between revisions of "Part:BBa K1548001"

Revision as of 01:24, 18 October 2014

Hsp60 promoter (M. bovis) and RBS (mycobacteriophage TM4)

Hsp60 promoter (M. bovis) Strong constitutive promoter from M. bovis and fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.

References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4. Sequence and Features Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 202
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K1548001 short</partinfo>
-This is the hsp60 promoter from M. bovis. It has also been shown to work in certain Propionibacterium. Due to this, we hypothesized that it would work in P. Acnes.
+Hsp60 promoter (M. bovis)
+Strong constitutive promoter from ''M. bovis'' and fused to a strong RBS from TM4 bacteriophage. This hybrid promoter/RBS has been shown to function in a variety of mycobacteria. (1) It is expected to function in P. acnes a closely-related propionibacterium.
+References: 1. Oldfield LM, Hatfull GF. Mutational Analysis of the Mycobacteriophage BPs Promoter PR Reveals Context-Dependent Sequences for Mycobacterial Gene Expression. J Bacteriol. 2014 Oct 15;196(20):3589-97. doi: 10.1128/JB.01801-14. Epub 2014 Aug 4.
+Sequence and Features
 <!-- Add more about the biology of this part here
 ===Usage and Biology===