Difference between revisions of "Part:BBa K1431812:Experience"

(→‎Applications of BBa_K1431812)
(→‎Applications of BBa_K1431812)
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===Applications of BBa_K1431812===
 
===Applications of BBa_K1431812===
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After we constructed this part, it was tranformed into BL21 & DH5α to test expression effeciency. In the meantime, we did a comparison between BBa_K1431812 and a part of which promoter is changed to BBa_J23106(weak).
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'''After construction, this part was transformed into BL21 & DH5α to test expression efficiency. In the meantime, we made a comparison between BBa_K1431812 and a part whose promoter is to BBa_J23106(weak).'''
  
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We spread plate at 10:00 on 15th, Oct. Following are pictures which were taken every several hours after placing the plates into a incubator.
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We spread plate at 10:00 on 15th, Oct. Following are pictures which were taken several hours after placing the plates into a incubator.
  
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JA is J23100,JB is J23106.
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JA equals to J23100, and JB equals to J23106.
  
 
At 00:30 (16th,Oct.)
 
At 00:30 (16th,Oct.)
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'''From observation, expression in BL21 is stronger than that in DH5α, and the part only differs in promoter with J23106 unexpectedly is stronger.'''
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'''With observation, expression in BL21 is stronger than that in DH5α, and the part with promoter J23106 unexpectedly is stronger.'''
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:24, 18 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1431812

After construction, this part was transformed into BL21 & DH5α to test expression efficiency. In the meantime, we made a comparison between BBa_K1431812 and a part whose promoter is to BBa_J23106(weak).

We spread plate at 10:00 on 15th, Oct. Following are pictures which were taken several hours after placing the plates into a incubator.

JA equals to J23100, and JB equals to J23106.

At 00:30 (16th,Oct.)

https://static.igem.org/mediawiki/parts/2/2e/SUSTC-Shenzhen_Characterization_K09_0：30.png


At 1:50 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_1_50.png


At 3:00 (16th,Oct.)

SUSTC-Shenzhen_Characerization_K09_3.png


At 6:00 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_6.png


At 7:40 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_7_40.png


At 8:00 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_9.png


At 10:00 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_10.png


At 11:20 (16th,Oct.)

SUSTC-Shenzhen_Characterization_K09_11_20.png


Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

SUSTC-Shenzhen_K09_L-1.jpg


At 02:45 (17th,Oct.)

SUSTC-Shenzhen_K09_L-2.jpg


At 04:55 (17th,Oct.)

SUSTC-Shenzhen_K09_L-3.jpg


At 22:40 (17th,Oct.)

SUSTC-Shenzhen_K09_L-4.jpg


With observation, expression in BL21 is stronger than that in DH5α, and the part with promoter J23106 unexpectedly is stronger.

User Reviews

UNIQ32bca937af930f0a-partinfo-00000000-QINU UNIQ32bca937af930f0a-partinfo-00000001-QINU