Difference between revisions of "Part:BBa K1491033"
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T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell. | T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell. | ||
| + | <hr> | ||
| + | This plasmid was used in a two plasmid system to detect estrogen in water. | ||
| + | <center>https://static.igem.org/mediawiki/2014/1/11/Sensor.png</center> | ||
| + | <hr> | ||
| − | <p>The sensor final sensor system contains a YFP coding region to report the amount of T7 RNA Polymerase that is produced. The following graphs show the fluorescence of the system and its controls before and after the YFP coding region was added in Top10 cells. Two graphs are shown because of the high magnitude of RFU in the RNAP sample after YFP was added.</p> | + | <p>The sensor final sensor system contains a YFP coding region to report the amount of T7 RNA Polymerase that is produced. The following graphs show the fluorescence of the system and its controls before and after the YFP coding region was added in Top10 cells. Two graphs are shown because of the high magnitude of RFU in the RNAP sample after YFP was added. The following graphs show the amount of fluorescence in the red channel, so RFU is indicative of the amount of RFP that was synthesized in the two plasmid system.</p> |
<center>https://static.igem.org/mediawiki/2014/e/e3/Beforeafteryfp.png</center> | <center>https://static.igem.org/mediawiki/2014/e/e3/Beforeafteryfp.png</center> | ||
Latest revision as of 01:21, 18 October 2014
T7 promoter-RBS-RFP-T7 Terminator
T7 Promoter with a RBS (BBa_S04423), RFP (BBa_E1010) and a T7 terminator (BBa_B0016). Allows RFP to be transcribed and translated when T7 RNA polymerase is present in the cell.
This plasmid was used in a two plasmid system to detect estrogen in water.
The sensor final sensor system contains a YFP coding region to report the amount of T7 RNA Polymerase that is produced. The following graphs show the fluorescence of the system and its controls before and after the YFP coding region was added in Top10 cells. Two graphs are shown because of the high magnitude of RFU in the RNAP sample after YFP was added. The following graphs show the amount of fluorescence in the red channel, so RFU is indicative of the amount of RFP that was synthesized in the two plasmid system.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 763
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 604
Illegal AgeI site found at 716 - 1000COMPATIBLE WITH RFC[1000]