Difference between revisions of "Part:BBa K1431813:Experience"

 
(→‎Applications of BBa_K1431813)
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===Applications of BBa_K1431813===
 
===Applications of BBa_K1431813===
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After we constructed this part, it was tranformed into BL21 & DH5α to test expression effeciency. In then meanwhile, we did a comparison between BBa_K1431812 and of which part promoter is changed to BBa_J23106(weak).
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We spread plate at 10:00 on 15th, Oct. Following are pictures which was taken every several hours after placing the plates into a incubator.
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JA is J23100,JB is J23106.
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At 00:30 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/9/9b/K11-1.png</center>
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At 1:50  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/2/22/K11-2.png</center>
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At 3:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/8/84/K11-3.png</center>
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At 6:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f8/K11-4.png</center>
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At 7:40  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/2/25/K11-5.png</center>
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At 8:00  (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/cb/K11-6.png</center>
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At 10:00 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/d/dd/K11-7.png</center>
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At 11:20 (16th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/c/c4/K11-8.png</center>
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Then we isolated single colonies from the plates.
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At 00:50 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/5/52/K11-1.jpg</center>
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At 02:45 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/f/f9/K11-2.jpg </center>
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At 04:55 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/3/3f/K11-3.jpg </center>
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At 22:40 (17th,Oct.)
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<center>https://static.igem.org/mediawiki/parts/4/4a/K11-4.jpg </center>
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'''Unfortunately,  BBa_K1431813 didn't grow and there isn't any color on plates or in broth! '''<br>
  
 
===User Reviews===
 
===User Reviews===

Revision as of 01:05, 18 October 2014


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1431813

After we constructed this part, it was tranformed into BL21 & DH5α to test expression effeciency. In then meanwhile, we did a comparison between BBa_K1431812 and of which part promoter is changed to BBa_J23106(weak).

We spread plate at 10:00 on 15th, Oct. Following are pictures which was taken every several hours after placing the plates into a incubator.

JA is J23100,JB is J23106.

At 00:30 (16th,Oct.)

K11-1.png


At 1:50 (16th,Oct.)

K11-2.png


At 3:00 (16th,Oct.)

K11-3.png

At 6:00 (16th,Oct.)

K11-4.png


At 7:40 (16th,Oct.)

K11-5.png


At 8:00 (16th,Oct.)

K11-6.png


At 10:00 (16th,Oct.)

K11-7.png


At 11:20 (16th,Oct.)

K11-8.png


Then we isolated single colonies from the plates.

At 00:50 (17th,Oct.)

K11-1.jpg


At 02:45 (17th,Oct.)

K11-2.jpg


At 04:55 (17th,Oct.)

K11-3.jpg


At 22:40 (17th,Oct.)

K11-4.jpg


Unfortunately, BBa_K1431813 didn't grow and there isn't any color on plates or in broth!

User Reviews

UNIQ141e47ccbf162c69-partinfo-00000000-QINU UNIQ141e47ccbf162c69-partinfo-00000001-QINU