Difference between revisions of "Part:BBa K1520008"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K1520008 short</partinfo>
 
<partinfo>BBa_K1520008 short</partinfo>
 
 
Pcons2-rbs-lacI-Ter.  
 
Pcons2-rbs-lacI-Ter.  
 
It constantly produces lacI.
 
It constantly produces lacI.
  
 +
[[File:k152008.jpg]]
 +
 +
From left to right, Marker (DL10000), Pcons2, Pcons2-lacI, Pcons-lacI-Ter.
 +
 +
Primers used in PCR are VF2 and VR
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 01:03, 18 October 2014

Pcons2-rbs-lacI-Ter Pcons2-rbs-lacI-Ter. It constantly produces lacI.

K152008.jpg

From left to right, Marker (DL10000), Pcons2, Pcons2-lacI, Pcons-lacI-Ter.

Primers used in PCR are VF2 and VR

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1208
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]