Difference between revisions of "Part:BBa K1351014"
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spaIFEG are coding for immunity to the antibiotic subtilin in Bacillus subtilis ATCC6633.  
 
spaIFEG are coding for immunity to the antibiotic subtilin in Bacillus subtilis ATCC6633.  
 
spaI: Lipoprotein, spaFEG: ABC-Transporter
 
spaI: Lipoprotein, spaFEG: ABC-Transporter
 +
 +
This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:
 +
 +
{|
 +
|prefix with EcoRI, NotI, XbaI, SD and NgoMIV:
 +
|<span style="color:blue">GAATTC</span><span style="color:green">GCGGCCGC</span>T<span style="color:red">TCTAGA</span>GT<u>AAGGAGG</u>A<span style="color:orange">GCCGGC</span>
 +
|-
 +
|suffix with AgeI, SpeI, NotI and PstI:
 +
|<span style="color:orange">ACCGGT</span><u>TAA</u>T<span style="color:red">AC<u>TAG</u><u>T</u></span><u>A</u><span style="color:green"><u>G</u>CGGCCG</span><span style="color:blue">CTGCAG</span>
 +
|}
 +
Sites of restriction enzymes generating compatible overhangs have the same color:
 +
 +
<span style="color:blue">EcoRI</span> and <span style="color:blue">PstI</span> in blue, <span style="color:green">NotI</span> in green, <span style="color:red">XbaI</span> and <span style="color:red">SpeI</span> in red, <span style="color:orange">NgoMIV</span> and <span style="color:orange">AgeI</span> in orange. Shine-Dalgarno sequence and stop codons are underlined.
  
 
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 
This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Revision as of 01:00, 18 October 2014

spaIFEG Subtilin (Immunity)

spaIFEG are coding for immunity to the antibiotic subtilin in Bacillus subtilis ATCC6633. spaI: Lipoprotein, spaFEG: ABC-Transporter

This part was generated in a modified version of RFC25, where a strong Shine Dalgarno Sequence (SD) is included, and has the following prefix and suffix:

prefix with EcoRI, NotI, XbaI, SD and NgoMIV: GAATTCGCGGCCGCTTCTAGAGTAAGGAGGAGCCGGC
suffix with AgeI, SpeI, NotI and PstI: ACCGGTTAATACTAGTAGCGGCCGCTGCAG

Sites of restriction enzymes generating compatible overhangs have the same color:

EcoRI and PstI in blue, NotI in green, XbaI and SpeI in red, NgoMIV and AgeI in orange. Shine-Dalgarno sequence and stop codons are underlined.

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 722
    Illegal BglII site found at 1470
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1737
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1017
    Illegal SapI site found at 1297
    Illegal SapI.rc site found at 2020