Difference between revisions of "Part:BBa K1520016"
Line 4: Line 4:
 
Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter. The luxR is expressed constantly and it produces lacI when there is enough AHL.
 
Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter. The luxR is expressed constantly and it produces lacI when there is enough AHL.
  
 +
[[File:k1520016.jpg]]
 +
 +
From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter
 +
 +
The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 00:49, 18 October 2014

Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter

Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter. The luxR is expressed constantly and it produces lacI when there is enough AHL.

File:K1520016.jpg

From left to right, Marker(1Kb), Pcons2-rbs-luxR-Ter, Pcons2-rbs-luxR-Ter-Prlux-rbs-lacI-Ter

The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2215
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 985