Difference between revisions of "Part:BBa K1520004"
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[[File:K1520004.jpg]] | [[File:K1520004.jpg]] | ||
| + | From left to right M：Marker（DL2000）, a: Prlux, Prlux-rbs-lacI, Prlux-rbs-lacI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers. | ||
Primers used in PCR are VF2 and VR | Primers used in PCR are VF2 and VR | ||
Revision as of 00:40, 18 October 2014
Prlux-rbs-lacI-Ter
Prlux-rbs-lacI-Ter. It worked out an expression of lacI in the presence of luxR and AHL.
From left to right M：Marker（DL2000）, a: Prlux, Prlux-rbs-lacI, Prlux-rbs-lacI-T. The substances of electrophoresis are made from PCR of plasmids with VF2 and VR as their primers. Primers used in PCR are VF2 and VR
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1228
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]