Difference between revisions of "Part:BBa K1520024"

Revision as of 23:59, 17 October 2014

Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter

Plasmid in positive feedback verification: Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.

The blue line in the picture shows the fluorescence intensity of the bacteria with this plasmid, which serves as a control in our experiment. The yellow line and purple line show the fluorescence intensity of the bacteria with different positive feedback plasmids.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 2380
Illegal NheI site found at 2403
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1208
Illegal BglII site found at 2222
Illegal BglII site found at 4818
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 3358

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1520024 short</partinfo>
@@ Line 6: / Line 5: @@
 Pcons2-rbs-lacI-Ter-Plac-rbs-luxI-Ter-Pcons2-rbs-luxR-Ter-Prlux-rbs-rfp-rbs-luxI-rbs-luxR-Ter
 It is a positive feedback plasmid using luxI and luxR as key to feedback. Introducing IPTG will produce rfp, luxI and luxR, and luxI together with luxR will work back to enhance production of rfp.
+[[File:positive feedback.jpg]]
+The blue line in the picture shows the fluorescence intensity of the bacteria with this plasmid, which serves as a control in our experiment. The yellow line and purple line show the fluorescence intensity of the bacteria with different positive feedback plasmids.