Difference between revisions of "Part:BBa K1463602"

Revision as of 23:55, 17 October 2014

FliC with RBS and J23116 promoter

FliC with B0032 and B0034 RBS under the control of J23116 promoter.

This composite part was made by inserting a synthesised double-stranded oligonucleotide containing J23116 promoter and B0032 into K1463601 (fliC and B0034 RBS).

As shown in Figure 1E and 1F, this construct was able to restore swimming in a fliC knockout strain to ~75% of wild-type levels. These results are also shown in Figure 2.

Flic Motility Swarm Assay

Figure 1: FliC Swarm Motility Assays.
(A) DS941, (B) DS941 ΔfliC,
(C) DS941 ΔfliC + pSB1C3 fliC (no promoter), (D) DS941 ΔfliC + J23100 (mutant promoter) fliC,
(E) DS941 ΔfliC + J23116-fliC(1), (F) DS941 ΔfliC + J23116-fliC(2),
(G) DS941 ΔfliC + J23106-fliC(1), (H) DS941 ΔfliC + J23106-fliC(2)

Figure 2 - FliC Motility Histogram

For more information on the biobrick and methods used go to http://2014.igem.org/wiki/index.php?title=Team:Glasgow/Project/Mobility_Proteins#fliC

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1306
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 383
Illegal AgeI site found at 791
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 [https://parts.igem.org/wiki/index.php?title=Part:BBa_K1463600 FliC] with [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0032 B0032] and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0034 B0034] RBS under the control of [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23116 J23116] promoter.
-<br> This composite part was made by inserting synthesised oligonucleotides containing [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23116 J23116] promoter and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0032 B0032] into [https://parts.igem.org/Part:BBa_K1463601 K1463601] (fliC and B0034 RBS).
+<br> This composite part was made by inserting a synthesised double-stranded oligonucleotide containing [https://parts.igem.org/wiki/index.php?title=Part:BBa_J23116 J23116] promoter and [https://parts.igem.org/wiki/index.php?title=Part:BBa_B0032 B0032] into [https://parts.igem.org/Part:BBa_K1463601 K1463601] (fliC and B0034 RBS).
 <br>As shown in Figure 1E and 1F, this construct was able to restore swimming in a fliC knockout strain to ~75% of wild-type levels. These results are also shown in Figure 2.