Difference between revisions of "Part:BBa K1500014"

Latest revision as of 23:33, 17 October 2014

paroF->MCherry-MutH(E56A)

This biobrick contains mutH(E56A) and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH(E56A) and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached (florescence will decrease). mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1144
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1346
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1500014 short</partinfo>
-This biobrick contains mutH() and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH() and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached. mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.
+This biobrick contains mutH(E56A) and mCherry under the control of the tyrosine-sensitive promoter paroF. Unless the E. coli cell produces very high levels of tyrosine, the mutagenizing protein mutH(E56A) and mCherry will be produced. The mCherry is used as a reporter to indicate when this maximum tyrosine production is reached (florescence will decrease). mutH should cause genomic mutations that should result in the cell eventually producing high levels of tyrosine.
 <!-- Add more about the biology of this part here